Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Defining key criteria for microhaplotype locus selection in forensic genetics: Progress and recommendations by the Microhaplotype Working Group.

Forensic science international. Genetics·2026
Same author

Microhaplotypes in forensic genetics: From exploration to application in degraded DNA specimens.

Forensic science international. Genetics·2025
Same author

CYP2C gene polymorphisms in North African populations.

Molecular biology reports·2024
Same author

Genetic diversity of North African populations in the 17q21 genomic region.

Mammalian genome : official journal of the International Mammalian Genome Society·2024
Same author

Making inroads to precision medicine for the treatment of autoimmune diseases: Harnessing genomic studies to better diagnose and treat complex disorders.

Cambridge prisms. Precision medicine·2024
Same author

A Distinctive Pattern of Diversity for the <i>TAS2R38</i> Gene in North Africa.

Human biology·2023
Same journal

Rapid DNA analysis for automated processing and interpretation of low DNA content samples.

Investigative genetics·2016
Same journal

Y-chromosome phylogeographic analysis of the Greek-Cypriot population reveals elements consistent with Neolithic and Bronze Age settlements.

Investigative genetics·2016
Same journal

Tracing Jomon and Yayoi ancestries in Japan using ALDH2 and JC virus genotype distributions.

Investigative genetics·2016
Same journal

Early modern human dispersal from Africa: genomic evidence for multiple waves of migration.

Investigative genetics·2015
Same journal

Erratum to: 'Mutiny on the Bounty': the genetic history of Norfolk Island reveals extreme gender-biased admixture.

Investigative genetics·2015
Same journal

'Mutiny on the Bounty': the genetic history of Norfolk Island reveals extreme gender-biased admixture.

Investigative genetics·2015
See all related articles

Related Experiment Video

Updated: Apr 16, 2026

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
10:27

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

Published on: November 10, 2015

12.2K

Criteria for selecting microhaplotypes: mixture detection and deconvolution.

Kenneth K Kidd1, William C Speed1

  • 1Department of Genetics, Yale University School of Medicine, 333 Cedar Street, PO Box 208005, New Haven, CT 06520-8005 USA.

Investigative Genetics
|March 10, 2015
PubMed
Summary
This summary is machine-generated.

Microhaplotypes, short DNA segments with multiple SNPs, offer powerful forensic mixture detection. Even five microhaplotype loci with an average effective number of alleles (Ae) above 3.0 can achieve over 95% mixture detection probability.

Keywords:
DNA mixturesForensic identificationMicrohaplotypePopulation genetics

More Related Videos

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER
14:06

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Published on: June 23, 2012

15.9K
Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

14.2K

Related Experiment Videos

Last Updated: Apr 16, 2026

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
10:27

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

Published on: November 10, 2015

12.2K
Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER
14:06

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Published on: June 23, 2012

15.9K
Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

14.2K

Area of Science:

  • Forensic Science
  • Population Genetics
  • Molecular Biology

Background:

  • DNA sequencing is emerging as a standard forensic typing method.
  • Microhaplotypes are defined as short DNA segments (e.g., 200 bp) containing multiple single nucleotide polymorphisms (SNPs) that collectively form a multiallelic locus.
  • These markers are valuable for forensic applications, particularly in identifying complex DNA mixtures.

Purpose of the Study:

  • To evaluate the utility of microhaplotypes for forensic DNA mixture detection.
  • To establish a method for ranking microhaplotype loci based on their informativeness.

Main Methods:

  • Utilized the concept of the effective number of alleles (Ae) to quantify the informativeness of microhaplotype loci.
  • Calculated the probability of detecting mixtures based on varying Ae values.
  • Modeled the cumulative mixture detection probabilities using multiple microhaplotype loci.

Main Results:

  • The effective number of alleles (Ae) provides a metric to rank microhaplotype loci by their discriminatory power.
  • A high probability of detecting three or more alleles in a mixture occurs when all alleles are equally frequent.
  • Testing as few as five microhaplotype loci with average Ae values slightly above 3.0 can yield cumulative mixture detection probabilities exceeding 95%.

Conclusions:

  • Microhaplotypes with Ae values greater than 3 are highly beneficial for routine forensic casework.
  • While 3-SNP microhaplotypes can meet this criterion, 4-SNP microhaplotypes frequently exceed Ae values of 4, offering enhanced utility.