[Study on quality standard for Humulus scandens].

Area of Science:

• Pharmacognosy and Phytochemistry
• Analytical Chemistry
• Quality Control

Context:

• Humulus scandens is utilized in traditional medicine, necessitating standardized quality assessment.
• Existing quality control methods for Humulus scandens require refinement for accuracy and reliability.

Purpose:

• To establish a comprehensive quality standard for Humulus scandens.
• To develop and validate analytical methods for the identification and quantification of key active compounds, luteoloside and cosmosiin.
• To ensure the consistent quality and safety of Humulus scandens for medicinal use.

Summary:

• Microscopic analysis confirmed characteristic features of Humulus scandens.
• Thin-layer chromatography (TLC) and High-Performance Liquid Chromatography (HPLC) methods were optimized for compound analysis.
• HPLC analysis of 16 batches revealed luteoloside content ranging from 0.015% to 0.651% (avg. 0.148%) and cosmosiin from 0.003% to 0.118% (avg. 0.036%).
• Validated methods demonstrated excellent linearity, recovery rates (100.5% for luteoloside, 98.5% for cosmosiin), and reliability.

Impact:

• Provides a robust framework for the quality control of Humulus scandens.
• Ensures the efficacy and safety of herbal medicinal products derived from Humulus scandens.
• Facilitates regulatory compliance and standardization within the pharmaceutical industry.