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Updated: Apr 16, 2026
Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells
Published on: July 22, 2013
A ratiometric fluorescent molecular probe for visualization of mitochondrial temperature in living cells
Mitsumasa Homma1, Yoshiaki Takei, Atsushi Murata
1Department of Life Science & Medical Bioscience, Graduate School of Advanced Science & Engineering, Waseda University, TWins 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan. takeoka@waseda.jp.
Abstract:
Mitochondrial thermodynamics is the key to understand cellular activities related to homeostasis and energy balance. Here, we report the first ratiometric fluorescent molecular probe (Mito-RTP) that is selectively localized in the mitochondria and visualize the temperature. We confirmed that Mito-RTP could work as a ratiometric thermometer in a cuvette and living cells.
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