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An Efficient Method for Adenovirus Production
Published on: June 10, 2021
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Biobased monoliths for adenovirus purification
Cláudia S M Fernandes1, Bianca Gonçalves1, Margarida Sousa1,2
1†UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
ACS Applied Materials & Interfaces
|March 11, 2015
Summary
This study introduces biodegradable biopolymer monoliths for purifying adenoviruses, crucial for vaccines and gene therapy. A chitosan-based monolith achieved 100% adenovirus recovery, marking a breakthrough in bioprocessing.
Area of Science:
- Biotechnology
- Materials Science
- Virology
Background:
- Adenoviruses are key for vaccine development and gene therapy, necessitating efficient purification methods.
- Current purification often relies on synthetic polymers, limiting sustainability.
- Biopolymers offer a biodegradable and potentially more sustainable alternative for bioprocessing matrices.
Purpose of the Study:
- To explore biopolymer-based monoliths for adenovirus purification using clean technologies.
- To develop disposable and biodegradable matrices for bioprocessing viral particles.
- To assess the performance of these novel biopolymer monoliths in adenovirus purification.
Main Methods:
- Production of 20 monoliths from chitosan, agarose, and dextran at -20 °C and -80 °C.
- Characterization of monolith morphology, robustness, and permeability.
- Assessment of nonspecific binding of Adenovirus serotype 5 (Ad5).
- Functionalization with quaternary amine anion-exchange ligand and performance testing for Ad5 purification.
Main Results:
- Chitosan and poly(vinyl) alcohol (50:50) monoliths prepared at -80 °C exhibited superior properties.
- These specific monoliths demonstrated low nonspecific Ad5 binding.
- The optimized chitosan-based monolith achieved 100% recovery of bound Ad5 particles.
Conclusions:
- Biopolymer-based monoliths, processed via clean technologies, are effective for adenovirus purification.
- Chitosan and poly(vinyl) alcohol monoliths offer a promising, biodegradable alternative for bioprocessing.
- This work presents the first successful adenovirus purification using biopolymer monoliths from clean technologies.
