Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA fingerprints from hypervariable mitochondrial genotypes.

J C Avise1, B W Bowen, T Lamb

  • 1Department of Genetics, University of Georgia, Athens 30602.

Molecular Biology and Evolution
|May 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-cell transcriptomics identifies altered neutrophil dynamics and accentuated T-cell cytotoxicity in tobacco flavored e-cigarette exposed mouse lungs.

bioRxiv : the preprint server for biology·2025
Same author

PHYLOGEOGRAPHY OF THE SARDINES (SARDINOPS SPP.): ASSESSING BIOGEOGRAPHIC MODELS AND POPULATION HISTORIES IN TEMPERATE UPWELLING ZONES.

Evolution; international journal of organic evolution·2017
Same author

Population genetics and evolution of the mangrove rivulus Kryptolebias marmoratus, the world's only self-fertilizing hermaphroditic vertebrate.

Journal of fish biology·2015
Same author

Reefs and islands of the Chagos Archipelago, Indian Ocean: why it is the world's largest no-take marine protected area.

Aquatic conservation : marine and freshwater ecosystems·2014
Same author

The slider turtle as an environmental sentinel: multiple tissue assays using flow cytometric analysis.

Ecotoxicology (London, England)·2013
Same author

Common misconceptions in molecular ecology: echoes of the modern synthesis.

Molecular ecology·2012

Mitochondrial DNA analysis revealed unique genetic fingerprints in menhaden fish and chuckwalla lizards due to high variation. These mitochondrial DNA fingerprints offer powerful tools for genetic identification and maternity assessment.

Area of Science:

  • Genetics
  • Molecular Biology
  • Zoology

Background:

  • Mitochondrial DNA (mtDNA) analysis is crucial for understanding genetic diversity.
  • Previous studies have explored restriction-fragment polymorphisms but often with lower variation.
  • Assessing genetic variation in distinct species like fish and reptiles is important for evolutionary studies.

Purpose of the Study:

  • To investigate the extent of genetic variation in mitochondrial DNA of menhaden fish and chuckwalla lizards.
  • To determine if mitochondrial DNA restriction-fragment polymorphisms can generate unique genetic "fingerprints" for individuals.
  • To explore the potential applications of these mitochondrial DNA fingerprints in genetic assessments, particularly for maternity.

Main Methods:

  • Restriction-fragment length polymorphism (RFLP) analysis was performed on mitochondrial DNA samples.

Related Experiment Videos

  • Surveys were conducted on populations of menhaden fish (Brevoortia tyrannus/patronus complex) and chuckwalla lizards (Sauromalus obesus).
  • Gel electrophoresis was used to visualize and compare restriction fragment patterns.

    • Main Results:

    • Exceptionally high levels of genetic variation were observed in both species.
    • Variation was attributed to differences in both mitochondrial DNA size and restriction sites.
    • High probabilities of unique genotypes (0.998 for fish, 0.983 for lizards) indicate distinct "fingerprints" for most individuals.
    • Mitochondrial DNA fingerprints were empirically distinct from nuclear DNA fingerprints.

    Conclusions:

    • Mitochondrial DNA analysis reveals substantial genetic variation in menhaden fish and chuckwalla lizards.
    • The resulting unique mitochondrial DNA "fingerprints" are highly effective for individual identification within these species.
    • These findings highlight the utility of mitochondrial DNA fingerprinting for applications such as genetic maternity assessment.