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Antimicrobial lubricant formulations containing poly(hydroxybenzene)-trimethoprim conjugates synthesized by

Idalina Gonçalves1, Cláudia M Botelho, Ana Teixeira

  • 1CEB - Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal.

Applied Microbiology and Biotechnology
|March 13, 2015
PubMed

Insights

Researchers developed novel antimicrobial poly(hydroxybenzene)-trimethoprim conjugates using an enzymatic method. These conjugates effectively inhibited bacterial growth, showing significant reductions in Escherichia coli and Staphylococcus epidermidis.

Area of Science:

  • Biomaterials Science
  • Enzymatic Synthesis
  • Antimicrobial Agents

Background:

  • Antimicrobial resistance necessitates novel therapeutic strategies.
  • Poly(hydroxybenzene) structures offer potential for functionalization.
  • Enzymatic synthesis provides a green chemistry approach for conjugate preparation.

Purpose of the Study:

  • To develop poly(hydroxybenzene)-trimethoprim conjugates using tyrosinase.
  • To evaluate the antimicrobial efficacy of these conjugates.
  • To propose an enzymatic strategy for producing antimicrobial conjugates.

Main Methods:

  • Enzymatic oxidation of methylparaben catalyzed by tyrosinase.
  • Formation of poly(hydroxybenzene) and poly(hydroxybenzene)-trimethoprim conjugates.
  • Incorporation of conjugates into hydroxyethyl cellulose/glycerol mixtures.
  • Antimicrobial activity testing against Escherichia coli and Staphylococcus epidermidis.

Main Results:

  • Enzymatic oxidation of methylparaben yielded poly(hydroxybenzene).
  • Poly(hydroxybenzene)-trimethoprim conjugates were successfully synthesized.
  • Conjugates demonstrated high efficacy in reducing bacterial suspensions by 96-97% within 24 hours.
  • Incorporation into hydroxyethyl cellulose/glycerol mixtures was achieved.

Conclusions:

  • A novel enzymatic strategy for preparing antimicrobial poly(hydroxybenzene)-antibiotic conjugates was established.
  • The synthesized conjugates show potent antimicrobial activity.
  • This approach holds promise for biomedical applications requiring antimicrobial properties.

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