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Updated: Apr 16, 2026

Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set
Published on: December 8, 2009
Gene Transfer into Pluripotent Stem Cells via Lentiviral Transduction
Ortwin Naujok1, Ulf Diekmann2, Matthias Elsner2
1Institute of Clinical Biochemistry, Hannover Medical School, 30625, Hannover, Germany. naujok.ortwin@mh-hannover.de.
Abstract:
Recombinant lentiviral vectors are powerful tools to stably manipulate human pluripotent stem cells. They can be used to deliver ectopic genes, shRNAs, miRNAs, or any possible genetic DNA sequence into diving and nondividing cells. Here we describe a general protocol for the production of self-inactivating lentiviral vector particles and their purification to high titers by either ultracentrifugation or ultrafiltration. Next we provide a basic procedure to transduce human pluripotent stem cells and propagate clonal cell lines.
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