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Detection of Class I Integrons in Staphyloacoccus aurous Isolated From Clinical Samples

Emad Yahaghi1, Abbas Ali Imani Fooladi2, Mohsen Amin3

  • 1Department of Microbiology, Science and Research Branch, Islamic Azad University, Damghan, IR Iran.

Iranian Red Crescent Medical Journal
|March 13, 2015
PubMed
Abstract

Insights

Class 1 integrons were found in a limited frequency (1%) of Staphylococcus aureus strains from clinical samples in Tehran. This study highlights the presence of these resistance genes in Iranian S. aureus isolates.

Area of Science:

  • Medical Microbiology
  • Infectious Diseases
  • Molecular Biology

Background:

  • Staphylococcus aureus is a significant global pathogen responsible for various diseases and mortality.
  • Antibiotic resistance in S. aureus is a growing concern, exacerbated by the inappropriate use of antibiotics and the spread of resistance genes.
  • Integrons, such as class 1 integrons, are mobile genetic elements that contribute to antibiotic resistance by carrying gene cassettes.

Purpose of the Study:

  • To investigate the prevalence of class 1 integrons in Staphylococcus aureus strains from clinical samples in Iran for the first time.
  • To identify potential sources and demographic factors associated with S. aureus carrying class 1 integrons.

Main Methods:

  • A descriptive study was conducted on 200 S. aureus strains isolated from patients at Baqiyatallah Hospital, Tehran, in 2013.
  • Strains were confirmed using biochemical and serological tests.
  • Polymerase chain reaction (PCR) was employed to detect the presence of the class 1 integron gene.

Main Results:

  • Class 1 integrons were detected in 1% (two isolates) of the 200 S. aureus strains analyzed.
  • The majority of isolates were from male patients, predominantly in the 51-60 age group.
  • Wound samples yielded the highest number of strains, with frequent isolation from emergency and intensive care units.

Conclusions:

  • Class 1 integrons exhibit a limited frequency in Staphylococcus aureus strains isolated from clinical samples in Tehran.
  • These findings provide initial data on the prevalence of class 1 integrons in Iranian S. aureus, contributing to understanding antimicrobial resistance patterns.
Keywords:
Drug ResistancePolymerase Chain ReactionStaphylococcus aurous

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