Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vivo dedifferentiation of human epidermal cells.

Cell biology international·2007
Same author

What is in a word? No versus Yes differentially engage the lateral orbitofrontal cortex.

Emotion (Washington, D.C.)·2007
Same author

[Gene expression profile changes in oral verrucous carcinoma and oral squamous cell carcinoma].

Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology·2007
Same author

Morphology of critical nuclei in solid-state phase transformations.

Physical review letters·2007
Same author

Enhanced cooperative activation effect in the hydrolytic kinetic resolution of epoxides on [Co(salen)] catalysts confined in nanocages.

Angewandte Chemie (International ed. in English)·2007
Same author

[Construction of the three-dimensional finite element model of micro -implant -maxilla].

Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology·2007

Related Experiment Video

Updated: Apr 16, 2026

Author Spotlight: Effective Reuse of Polycarbonate Tubes for Extracellular Vesicle Isolation
02:36

Author Spotlight: Effective Reuse of Polycarbonate Tubes for Extracellular Vesicle Isolation

Published on: March 8, 2024

1.8K

[Protein enrichment based on large volume recycling injection].

Quanqing Zhang, Lei Zhang, Xiaodi Gao

    Se Pu = Chinese Journal of Chromatography
    |March 14, 2015
    PubMed
    Summary

    Detecting low-abundance proteins is crucial for understanding biological functions and diseases. This study introduces a novel large volume recycling injection method to significantly enhance the detection of these vital, yet scarce, biological proteins.

    More Related Videos

    Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
    11:12

    Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

    Published on: February 7, 2022

    13.0K
    Purification of High Yield Extracellular Vesicle Preparations Away from Virus
    07:15

    Purification of High Yield Extracellular Vesicle Preparations Away from Virus

    Published on: September 12, 2019

    12.5K

    Related Experiment Videos

    Last Updated: Apr 16, 2026

    Author Spotlight: Effective Reuse of Polycarbonate Tubes for Extracellular Vesicle Isolation
    02:36

    Author Spotlight: Effective Reuse of Polycarbonate Tubes for Extracellular Vesicle Isolation

    Published on: March 8, 2024

    1.8K
    Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
    11:12

    Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

    Published on: February 7, 2022

    13.0K
    Purification of High Yield Extracellular Vesicle Preparations Away from Virus
    07:15

    Purification of High Yield Extracellular Vesicle Preparations Away from Virus

    Published on: September 12, 2019

    12.5K

    Area of Science:

    • Biochemistry
    • Analytical Chemistry
    • Proteomics

    Background:

    • Biological proteins exhibit vast differences in properties like size, hydrophobicity, and abundance.
    • The concentration disparity between high- and low-abundance proteins in biological fluids can exceed 11 orders of magnitude.
    • Crucial disease-related proteins are often present at very low concentrations, hindering their detection and study.

    Purpose of the Study:

    • To develop a new sample enrichment technology for improving the detection of low-abundance proteins.
    • To enhance the sensitivity of protein analysis in biological samples.
    • To establish a method for overcoming the challenge of low protein concentrations in complex biological matrices.

    Main Methods:

    • Development of a large volume recycling injection technique for sample enrichment.
    • Optimization of injection volume and number of injection cycles.
    • Application of the method to pork liver protein samples to assess enrichment efficiency.

    Main Results:

    • The large volume recycling injection method effectively improved the detection of low-abundance proteins.
    • Enrichment factors of 52 and 61 were achieved for proteins at specific retention times (11.38 min and 12.58 min) after 11 injections of 500 μL.
    • Enrichment factors closely approached theoretical values, demonstrating method efficacy.

    Conclusions:

    • The developed large volume recycling injection method is a valuable tool for enriching biological protein samples.
    • This technique significantly enhances the ability to detect low-abundance proteins, which are critical for biological and medical research.
    • The method shows promise for broader applications in analyzing complex biological samples.