Ozone preconditioning attenuates contrast-induced nephropathy in rats

Area of Science:

• Nephrology
• Experimental Medicine
• Biochemistry

Background:

• Contrast-induced nephropathy (CIN) is a significant complication following vascular interventions.
• Ozone therapy can induce tolerance to ischemic injury via ozone oxidative preconditioning (OOP).

Purpose of the Study:

• To investigate the protective effects of ozone oxidative preconditioning (OOP) against experimental contrast-induced nephropathy (CIN).

Main Methods:

• Wistar rats were divided into control, contrast media (CM), ozone (O3), and oxygen (O2) groups.
• CIN was induced by intravenous contrast agent. Ozone or oxygen was administered intraperitoneally for 5 days prior.
• Renal function, oxidative stress markers (MDA, NO), and renal histology were assessed 48 hours post-contrast.

Main Results:

• Contrast administration significantly increased BUN, creatinine, MDA, and tubular necrosis, while decreasing NO levels.
• OOP treatment notably reduced BUN, creatinine, MDA, and tubular necrosis compared to the CM group.
• The O3 group exhibited higher serum and renal nitric oxide (NO) and total antioxidant system levels than the CM group.

Conclusions:

• Ozone oxidative preconditioning (OOP) effectively attenuates experimental contrast-induced nephropathy (CIN).
• The renoprotective mechanism of OOP involves strengthening renal antioxidant capacity and maintaining nitric oxide levels.