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Classification of a Proteus penneri clinical isolate with a unique O-antigen structure to a new Proteus serogroup,

Małgorzata Siwińska1, Evgeniya A Levina2, Olga G Ovchinnikova3

  • 1Department of General Microbiology, Institute of Microbiology, Biotechnology and Immunology, University of Łódź, 90-237 Łódź, Poland.

Carbohydrate Research
|March 16, 2015
PubMed

Insights

A new Proteus serogroup, O80, has been identified. This serogroup is based on the unique O-antigen structure of Proteus penneri Br 114, a pathogen causing urinary tract infections.

Area of Science:

  • Microbiology
  • Bacteriology
  • Immunology

Background:

  • Proteus penneri is an opportunistic pathogen often causing severe urinary tract infections in immunocompromised individuals.
  • The strain P. penneri Br 114, isolated from a patient wound in Poland, exhibits significant swarming motility.
  • Understanding the serological diversity of P. penneri is crucial for diagnostics and epidemiology.

Purpose of the Study:

  • To characterize the O-antigen structure of P. penneri Br 114.
  • To determine if this strain represents a new Proteus serogroup.
  • To contribute to the classification of Proteus penneri.

Main Methods:

  • Serological analyses including ELISA and Western blotting.
  • Chemical analyses of the O-antigen.
  • (1)H and (13)C NMR spectroscopy for structural elucidation.

Main Results:

  • The O-antigen of P. penneri Br 114 possesses a unique linear pentasaccharide repeating unit.
  • The structure includes a partially O-acetylated amide of D-glucuronic acid with L-serine.
  • This unique structure supports the designation of a new serogroup, O80.

Conclusions:

  • The strain P. penneri Br 114 represents a novel Proteus serogroup, O80.
  • This finding expands the known serological landscape of Proteus penneri.
  • The identification of O80 is based on distinct O-antigen structural features.
Keywords:
Bacterial polysaccharide structureLipopolysaccharideO-antigenProteus penneriSerological classification

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