Structural studies on cell wall and matrix polysaccharides of Micrococcus luteus HB3.2.1

Matrix and cell wall polysaccharides of Micrococcus luteus HB3.2.1 were obtained. The following structures of the glycopolymers were established by compositional analysis and 1D and 2D NMR spectroscopy: →4)-ɑ-d-GalpNAc-(1→2)-l-2HO-Glt-(5→2)-d-Asn-(1→4)-ɑ-d-QuipNAc4N-(1→ PSI. where d-Asn indicates d-asparagine, l-2HO-Glt - l-2-hydroxy-glutaric acid. →2)-ɑ-d-Manp-(1→3)-ɑ-d-Manp-(1→6)-ɑ-d-Manp-(1→ PSII. →6)-ɑ-d-Glcp-(1→4)-β-d-ManpNAcA-(1→ PSIII. →6)-ɑ-d-Glcp-(1→4)-β-d-GlcpNAc3NAcA-(1→ PSIV. The structure of the PSI is unique among the bacterial polysaccharides. It is including non-sugar residues such as d-asparagine andl-2-hydroxy-glutaric acid in the main chain. The matrix polysaccharide (PSI) possesses a cytotoxic activity.