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Updated: Apr 16, 2026
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Purification of the Dendritic Filopodia-rich Fraction
Published on: May 2, 2019
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Endophilin A1 regulates dendritic spine morphogenesis and stability through interaction with p140Cap
Yanrui Yang1, Mengping Wei2, Ying Xiong3
11] State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Beijing 100101, China [2] CAS Center for Excellence in Brain Science, Chinese Academy of Sciences, Beijing 100101, China.
Cell Research
|March 17, 2015
Summary
Endophilin A1 plays a new role in dendritic spine development and function. This protein regulates p140Cap, impacting learning and memory in the brain.
Area of Science:
- Neuroscience
- Cell Biology
- Molecular Biology
Background:
- Dendritic spines are crucial for brain learning and memory.
- Endophilin A1 is known for presynaptic vesicle endocytosis.
Purpose of the Study:
- Investigate the role of endophilin A1 in postsynaptic dendritic spines.
- Identify downstream effectors and mechanisms involved in spine morphogenesis.
Main Methods:
- Immunolocalization of endophilin A1 in dendritic spines.
- Knockdown studies of endophilin A1 and p140Cap.
- Analysis of F-actin and cortactin distribution.
Main Results:
- Endophilin A1 localizes to dendritic spines and is essential for their development and function.
- p140Cap identified as a downstream effector of endophilin A1.
- Disruption of endophilin A1-p140Cap interaction impairs spine formation and maturation.
- Endophilin A1 regulates p140Cap, cortactin, and F-actin in spines, affecting spine stabilization.
Conclusions:
- Endophilin A1 has a novel postsynaptic function in dendritic spine morphogenesis and stabilization.
- Regulation of p140Cap by endophilin A1 is critical for synaptic function and plasticity.
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