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Updated: Apr 16, 2026

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons
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Simplified CLARITY for visualizing immunofluorescence labeling in the developing rat brain.

Huiyuan Zheng1, Linda Rinaman2

  • 1Department of Neuroscience, University of Pittsburgh, A210 Langley Hall, Pittsburgh, PA, 15260, USA.

Brain Structure & Function
|March 17, 2015
PubMed
Summary

A simplified CLARITY protocol transforms intact brain tissue into a nanoporous hydrogel, enabling detailed visualization of chemically identified neurons and their structures in developing rats. This method offers high-resolution insights into neural circuit development.

Failed At:

2026-06-19T13:44:22.357632+00:00

Keywords:
CLARITYImmunofluorescenceNeonatal

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