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Updated: Apr 16, 2026

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons
Published on: May 12, 2015
Huiyuan Zheng1, Linda Rinaman2
1Department of Neuroscience, University of Pittsburgh, A210 Langley Hall, Pittsburgh, PA, 15260, USA.
A simplified CLARITY protocol transforms intact brain tissue into a nanoporous hydrogel, enabling detailed visualization of chemically identified neurons and their structures in developing rats. This method offers high-resolution insights into neural circuit development.
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