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Related Experiment Videos

CD2 expression in murine B cell lineage.

H Yagita1, T Nakamura, J Asakawa

  • 1Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.

International Immunology
|January 1, 1989
PubMed
Summary

Murine CD2 is expressed on B cells during specific differentiation stages. This study confirms CD2 presence on peripheral B cells and B cell lines, indicating its role in B cell lineage development.

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Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • CD2 is a T cell surface glycoprotein crucial for T cell receptor-mediated immune responses.
  • Its expression and function in B cell lineage have been less understood.

Purpose of the Study:

  • To investigate the expression of CD2 in the murine B cell lineage.
  • To determine the stages of B cell differentiation where CD2 is present.

Main Methods:

  • Flow cytometry and immunoprecipitation studies using anti-murine CD2 monoclonal antibody (mAb).
  • Northern blot analysis to detect the CD2 transcript.
  • Analysis of various murine B cell populations, including peripheral B cells, B cell lines, bone marrow B cells, and pre-B cells.

Main Results:

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  • Cell surface expression of CD2 was detected on all peripheral B cells and B lineage cell lines.
  • A 1.3 kb murine CD2 transcript was identified in these B cells.
  • An identical 55-67 KD glycoprotein was precipitated from various T and B cell types, including B cell lymphomas.
  • CD2 expression was observed in the majority of bone marrow B cells and approximately half of pre-B cells.

Conclusions:

  • Murine CD2 is expressed on B cells at distinct stages of their differentiation.
  • These findings suggest a potential role for CD2 in B cell development and function beyond T cells.