Related Experiment Video
Updated: Apr 16, 2026
Protein Engineering by Yeast Surface Display
Published on: November 29, 2024
Surface display of highly-stable Desulfovibrio vulgaris carbonic anhydrase on polyester beads for CO2 capture
David O Hooks1, Bernd H A Rehm
1Institute of Fundamental Sciences, Massey University, Private Bag 11222, Palmerston North, New Zealand, d.o.hooks@massey.ac.nz.
Objectives:
To engineer a recombinant Escherichia coli to produce polyhydroxyalkanoate biopolymer beads displaying carbonic anhydrase (CA) from Desulfovibrio vulgaris str. "Miyazaki F" (DvCA).
Results:
The highest measured specific activity of the immobilised CA was 211 U/mg DvCA. The immobilised CA was thermostable, retaining 114 U/mg DvCA of activity after incubation at 90 °C for 1 h. Additionally, the immobilised CA tolerated 30 min incubations in a variety of pH conditions and was especially tolerant of alkaline conditions, retaining 131 U/mg DvCA of activity after pH 12 incubation.
Conclusion:
CA has a potential role in atmospheric CO2 mitigation strategies and the stability of the functionalised beads indicates suitability for use in industrial settings such as coal-fired power plants.
More Related Videos
08:00Author Spotlight: Standardizing the Development of Amine-Based Silica Composites as CO2 Adsorbents for Direct Air Capture
Published on: September 29, 2023
10:57Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
Published on: November 2, 2009
