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Effect of bone morphogenic protein-7 on the expression of epithelial-mesenchymal transition markers in silicosis
Yan Wang1, Gengxia Yang1, Zhonghui Zhu1
1Department of Occupational Health, Environmental Health School of Public Health, Capital Medical University, Beijing 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, China.
Abstract:
This study presented the effect of bone morphogenic protein-7 (BMP-7) inhibiting epithelial-mesenchymal transition (EMT) in silicosis model. In vivo, Wistar rats were exposed to silica by intratracheal instillation. Seven days later rats were treated with BMP-7. Rats were sacrificed at 15 and 30days after exposure of silica. The results demonstrated vimentin expression was down-regulated; and E-cadherin was up-regulated after intervention with BMP-7. The TGF-β expression and phosphorylation-p38 were lower in BMP-7 treated group than in silica group. In vitro, p38 MAPK/Snail signaling pathway was involved in the occurrence of EMT in A549 cells treated by silica. EMT was inhibited by BMP-7. The data showed BMP-7 inhibited EMT induced by silica associated with inhibition of p38 MAPK/Snail pathway.
