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Updated: Apr 16, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
Towards metal-mediated g-quartet analogues: 1,2,4-triazole nucleotides
Jamie M Withers1, Shane G Telfer, Vyacheslav V Filichev
1a Institute of Fundamental Sciences , Massey University , Palmerston North , New Zealand.
Abstract:
We proposed that metal-coordinating nucleotides could be used to control the assembly of G-quadruplexes through the formation of an artificial metal-centered quartet. Several guanine-rich DNA sequences containing 1,2,4-triazole-functionalized nucleotides were investigated. These oligonucleotides were designed to form quartets mediated by metal-triazole bonding both on the surface of and within the G-quadruplex core. In contrast to duplex studies in which 1,2,4-triazole nucleosides serve as a mimic of Watson-Crick base-pairs, our results show that these nucleosides are not suitable components of an artificial metal-centered quartet.
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