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Published on: June 28, 2014
Fluorous-Directed Clamping Stabilizes Triple-Helical DNA
Andrea Taladriz-Sender1,2, Michael Brazzill3, Jamie M Withers1,2
1Department of Pure Applied Chemistry, University of Strathclyde, Thomas Graham Building, 295 Cathedral Street, Glasgow G1 1XL, U.K.
None:
Here we present a strategy to stabilize DNA triplexes by the attachment of perfluorinated tails to the 3' and/or 5' end of oligodeoxyribonucleotides. The tails preferentially associate with one another via the "fluorous effect" without compromising sequence selectivity. The most stable complex resulted in a dramatic 14 °C increase in melting temperature at neutral pH. This orthogonal recognition approach broadens the applications of triplex DNA in diagnostics, therapy, and DNA nanotechnology.
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