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Updated: Apr 16, 2026

Genetic Incorporation of Biosynthesized L-dihydroxyphenylalanine DOPA and Its Application to Protein Conjugation
Published on: August 24, 2018
Dithiol-based modification of poly(dopamine): enabling protein resistance via short-chain ethylene oxide oligomers
Amit Vaish1, David J Vanderah, Lee J Richter
1National Institute of Standards and Technology (NIST) Center for Neutron Research, Gaithersburg, Maryland 20899-8313, USA.
Abstract:
We present a facile strategy to modify poly(dopamine) (PDA)-coated substrates. Using thiol-terminated short chain ethylene oxide oligomers (OEG) under aqueous conditions, we explore the creation of a model surface exhibiting resistance to nonspecific protein absorption (RPA) by engineering the surface properties of a PDA adlayer. Surprisingly, dithiol-terminated OEG molecules demonstrated significantly greater coverage on PDA surfaces than analogous monothiol molecules. Successful RPA is only achieved with dithiol-terminated OEGs.
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