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Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
Evolution and single-nucleotide polymorphisms in methicillin-resistant Staphylococcus aureus strains with reduced
Tetsuo Yamaguchi1, Shingo Suzuki2, Sakiko Okamura3
1Department of Microbiology, Tokyo Medical University, Tokyo, Japan Department of Infection Control, Tokyo Medical University Hospital, Tokyo, Japan tetsuo.yamaguchi@med.toho-u.ac.jp.
Abstract:
We obtained a series of methicillin-resistant Staphylococcus aureus isolates, including both daptomycin-susceptible strain TD1 and daptomycin-resistant strain TD4, from a patient. We determined the complete genome sequences of TD1 and TD4 using next-generation sequencing, and only four single-nucleotide polymorphisms (SNPs) were identified, one each in capB (E58K), rpoB (H481Y), lytN (I16V), and mprF (V351E). We determined that these four SNPs were sufficient to cause the strains to develop daptomycin, vancomycin, and rifampin resistance.
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