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Three-Dimensional Imaging of Tumor-Bearing Tissue Using the Iterative Bleaching Extends Multiplexity Approach

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High-throughput multiphoton-induced three-dimensional ablation and imaging for biotissues.

Chun-Yu Lin¹, Pei-Kao Li¹, Li-Chung Cheng²

¹Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan.

Biomedical Optics Express

|March 18, 2015

Summary

This study introduces a high-throughput multiphoton ablation system for rapid biotissue disruption. The technique efficiently ablates large areas with minimal thermal damage, enabling high-speed tissue processing and potential for whole bio-specimen molecular imaging.

Keywords:

(140.3390) Laser materials processing (170.1020) Ablation of tissue (190.4180) Multiphoton processes

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Published on: August 6, 2014

Area of Science:

Biomedical Engineering
Optical Physics
Microscopy

Background:

Multiphoton excitation enables deeper tissue penetration and reduced photodamage.
High-throughput laser ablation is crucial for efficient biological sample processing.
Axially-resolved imaging is essential for detailed analysis of complex tissues.

Purpose of the Study:

To develop and demonstrate a temporal focusing-based high-throughput multiphoton-induced ablation system.
To evaluate the system's efficiency and precision in biotissue disruption.
To explore its application in optical imaging of biological specimens.

Main Methods:

Utilized a temporal focusing-based system for multiphoton-induced ablation.
Employed axially-resolved widefield multiphoton excitation for precise targeting.
Quantified ablation rates and assessed photothermal damage in biotissues.

Main Results:

Achieved high-throughput, large-area laser ablation of biotissues with minimal collateral thermal damage.
Demonstrated a tissue processing rate of approximately 1.6 × 10^6 μm^3/s in chicken tendon.
Showcased the system's utility in optical imaging via iterative ablation and optical sectioning.

Conclusions:

The developed system offers efficient and precise biotissue disruption.
It minimizes photothermal damage, preserving surrounding tissue integrity.
The technology holds potential for high-throughput molecular imaging of entire biological specimens.