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Related Experiment Video

Updated: Apr 16, 2026

Fluorescence Lifetime Macro Imager for Biomedical Applications

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

Singlet oxygen phosphorescence lifetime imaging based on a fluorescence lifetime imaging microscope.

Wenming Tian¹, Liezheng Deng¹, Shengye Jin¹

¹†State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning 116023, China.

The Journal of Physical Chemistry. A

|March 18, 2015

Summary

Singlet oxygen phosphorescence (SOP) imaging feasibility was explored using a modified fluorescence lifetime imaging microscope (FLIM). Researchers developed a method to capture weak SOP signals, overcoming challenges like long imaging times for potential applications.

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Area of Science:

Biophysics
Photochemistry
Microscopy

Background:

Singlet oxygen phosphorescence (SOP) is an infrared emission from O2(a(1)Δg).
Capturing weak SOP signals requires specialized microscopy techniques.

Purpose of the Study:

To investigate the feasibility of singlet oxygen phosphorescence (SOP) lifetime imaging microscopy.
To adapt a fluorescence lifetime imaging microscope (FLIM) for SOP detection.

Main Methods:

A modified FLIM system was employed, incorporating a dichroic mirror to separate SOP signals from C60 photoluminescence (C60-PL).
Simultaneous confocal C60-PL and nonconfocal SOP imaging were achieved using laser-scanning mode.

Main Results:

The study identified infrared-incompatible scanners as an obstacle to confocal SOP imaging, suggesting infrared-compatible scanners as a solution.
Alternative methods for confocal SOP imaging, including stage-scanning mode with a pinhole, were proposed.
The exceptionally long imaging time for SOP was noted as a significant challenge, with point measurements proposed as a compromise.

Conclusions:

The modified FLIM system demonstrates the feasibility of SOP lifetime imaging.
Overcoming technical challenges, such as scanner compatibility and imaging time, is crucial for advancing SOP imaging applications.