Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrated proteomic and transcriptomic analyses reveal that the Rj4-mediated immunity network restricts soybean-rhizobia symbiosis.

BMC genomics·2025
Same author

Long-term follow-up of zevor-cel in patients with relapsed/refractory multiple myeloma.

Blood advances·2025
Same author

Explainable person-job recommendations: challenges, approaches, and comparative analysis.

Frontiers in artificial intelligence·2025
Same author

The efficacy of Kinesio taping combined with exercise therapy on patients with patellofemoral pain syndrome: A systematic review and meta-analysis.

Journal of back and musculoskeletal rehabilitation·2025
Same author

Correction: Predictive role of the geriatric nutritional risk index in all-cause and cardiovascular mortality among elderly patients with osteoarthritis.

BMC geriatrics·2025
Same author

Establishment and application of efficient protoplast isolation and transformation system from leaves of multi-genotype poplars.

Plant science : an international journal of experimental plant biology·2025

Related Experiment Video

Updated: Apr 16, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

21.3K

MicroRNA-derived fragment length polymorphism assay.

Xiaoping Xie1, Fang Tang2, Zhao Yang1

  • 1Dujiangyan Medical Center, Chengdu, Sichuan, 611830, China.

Scientific Reports
|March 21, 2015
PubMed
Summary
This summary is machine-generated.

This study introduces a precise method for quantifying microRNAs (miRNAs) using competitive PCR and synthetic RNA spike-ins. This breakthrough enables reliable miRNA detection for clinical diagnostics, overcoming previous limitations.

More Related Videos

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

34.1K
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

523

Related Experiment Videos

Last Updated: Apr 16, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

21.3K
Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

34.1K
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

523

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Clinical Diagnostics

Background:

  • MicroRNA (miRNA) research is shifting towards clinical applications.
  • Current miRNA detection methods lack the sensitivity and reliability needed for clinical use.
  • This gap hinders the translation of miRNA research into practical diagnostics.

Purpose of the Study:

  • To develop an absolute quantification method for microRNAs (miRNAs).
  • To establish a sensitive and reliable assay for miRNA detection in clinical samples.
  • To validate the assay's potential for diagnosing respiratory conditions.

Main Methods:

  • Absolute quantification of miRNAs using competitive PCR with synthetic RNA spike-ins in a single reaction.
  • Utilizing RNA spike-ins as dynamic RNA copy number standards for accurate measurement.
  • Employing capillary electrophoresis for size differentiation and precise miRNA quantification.
  • Validating detection limits across a 5-log range.

Main Results:

  • Achieved reproducible miRNA measurements with verifiable detection limits of 10-46 copies.
  • Demonstrated a 5-log dynamic detection range for miRNA quantification.
  • Successfully measured miRNAs in 168 human serum samples.
  • Showcased the diagnostic potential for bronchopneumonia and bronchiolitis.

Conclusions:

  • The developed assay provides a reliable and sensitive method for absolute miRNA quantification.
  • This technique overcomes the bottleneck of miRNA detection in clinical applications.
  • The assay shows significant promise as a diagnostic tool for respiratory diseases and beyond.