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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Updated: Apr 16, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Maternal plasma RNA sequencing.

Cees B M Oudejans¹

¹Department of Clinical Chemistry, VU University Medical Center, Amsterdam, The Netherlands.

Clinical Biochemistry

|March 21, 2015

Summary

This summary is machine-generated.

Maternal plasma RNA sequencing for pre-eclampsia needs optimization to match the specificity and sensitivity of DNA sequencing for trisomy. This review explores essential steps for improving RNA sequencing accuracy in prenatal diagnostics.

Keywords:

Maternal plasma Pre-eclampsia RNA sequencing

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Area of Science:

Reproductive biology
Genomics
Molecular diagnostics

Background:

Pre-eclampsia is a serious pregnancy complication.
Current diagnostic methods for pre-eclampsia have limitations.
Maternal plasma DNA sequencing for trisomy is highly accurate.

Purpose of the Study:

To identify key steps for optimizing maternal plasma RNA sequencing.
To achieve diagnostic performance comparable to DNA sequencing for trisomy.

Main Methods:

Review of current literature on RNA sequencing techniques.
Analysis of pre-eclampsia biomarkers in maternal plasma.
Comparison of RNA and DNA sequencing methodologies.

Main Results:

Specific RNA isolation and amplification protocols are crucial.
Bioinformatic pipelines require refinement for accurate RNA analysis.
Standardization of pre-eclampsia RNA sequencing is needed.

Conclusions:

Optimizing maternal plasma RNA sequencing requires advancements in sample processing and data analysis.
Achieving high specificity and sensitivity is feasible with targeted improvements.
Enhanced RNA sequencing could revolutionize pre-eclampsia diagnostics.