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Updated: Apr 16, 2026

Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue
Published on: May 5, 2022
Hypoacetylation in association with histone 3 modulation in human hepatocellular carcinoma
Chiung-Chi Cheng1, Yi-Hsiang Liu2, Yen-Chang Clark Lai3
1Department of Pathology, Chang Bing Show Chwan Memorial Hospital, Changhua County, Taiwan, R.O.C. Center for General Education, Providence University, Taichung City, Taiwan, R.O.C.
Background:
Aberrant histone deacetylase expression may cause imbalance between acetylation and deacetylation of histone and play roles in tumor transformation. We found that histone 3 was modulated in human hepatocellular carcinoma. We determined if histone 3 modulation is related to the aberrant expression of histone deacetylase.
Materials And Methods:
We analyzed human liver and hepatocellular carcinoma tissues and fibroblast and fibrosarcoma cell lines for the expression of histone 3, histone deacetylase 1 and acetylated histone 3 using immunohistochemistry, western blot and immunofluorescence.
Results:
Histone deacetylase 1 and histone 3 were more strongly detected in hepatocellular carcinoma tissue and fibrosarcoma cells than in liver tissues and fibroblast cells, respectively. However, acetylated histone 3 was more strongly expressed in normal liver and fibroblast cells and less expressed in hepatocellular carcinoma and fibrosarcoma cells.
Conclusion:
Histone deacetylase 1 overexpression and hypoacetylation of histone 3 might play critical roles in the modulation of histone 3 in human hepatocellular carcinoma.
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