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High-resolution chromatin dynamics during a yeast stress response
Assaf Weiner1, Tsung-Han S Hsieh2, Alon Appleboim1
1School of Computer Science and Engineering, The Hebrew University, Jerusalem 9190401, Israel; Institute of Life Sciences, The Hebrew University, Jerusalem 9190401, Israel.
Molecular Cell
|March 25, 2015
Summary
Histone modifications regulate gene transcription. During stress, yeast cells show increased histone modification complexity, revealing dynamic gene activation and repression sequences.
Area of Science:
- Molecular Biology
- Genetics
- Epigenetics
Background:
- Covalent histone modifications are crucial for eukaryotic transcription regulation.
- Existing knowledge suggests steady-state histone modification patterns reflect genomic processes like transcription.
Purpose of the Study:
- To map 26 histone modifications genome-wide in yeast under normal and stress conditions.
- To investigate changes in histone modification complexity and dynamics during transcriptional reprogramming.
Main Methods:
- Genome-wide mapping of 26 histone modifications in yeast.
- Analysis of histone modification patterns during diamide-induced stress response.
Main Results:
- Steady-state histone modification patterns exhibit limited combinatorial complexity.
- Stress response leads to a modest increase in combinatorial complexity due to transient rare histone states.
- Differences in modification kinetics, particularly slow methylation changes lagging acetylation, create these rare states.
Conclusions:
- Chromatin dynamics during massive transcriptional changes involve transient population of rare histone states.
- Modification dynamics reveal ordered sequences in gene activation and repression.
- Provides a comprehensive view of chromatin remodeling during stress-induced transcriptional reprogramming.
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