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Updated: Apr 15, 2026
Detection and Isolation of Apoptotic Bodies to High Purity
Published on: August 12, 2018
Detection of apoptosis using fluorescent probes.
Grishma Khanal1, Himali Somaweera, Meicong Dong
1Department of Chemistry and Biochemistry, Texas Tech University, 41061, Lubbock, TX, 79409, USA.
This chapter details apoptosis assay protocols for studying programmed cell death in healthy and diseased cells. Multiple cellular markers allow for analysis at various time points.
Area of Science:
- Cell Biology
- Molecular Biology
Background:
- Apoptosis, or programmed cell death, is a fundamental biological process crucial for development and tissue homeostasis.
- Dysregulation of apoptosis is implicated in numerous diseases, including cancer and autoimmune disorders.
Purpose of the Study:
- To provide a comprehensive overview of various apoptosis assay protocols.
- To enable researchers to study apoptosis at different stages using multiple cellular markers.
Main Methods:
- Detailed protocols for several apoptosis detection assays are presented.
- Methods focus on utilizing diverse cellular markers to monitor apoptotic events.
Main Results:
- The chapter offers a collection of established and validated apoptosis assay protocols.
- These protocols allow for robust detection and analysis of apoptosis.
Conclusions:
- The provided apoptosis assay protocols offer valuable tools for researchers.
- Accurate analysis of apoptosis is essential for understanding both normal physiology and disease pathogenesis.
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