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Self reactive delayed type hypersensitivity (DTH) induced in mice by syngeneic lymphoblasts.
Immunobiology
|February 1, 1985
Summary
Mice injected with concanavalin A (Con A)-induced lymphoblasts showed delayed type hypersensitivity (DTH) when challenged with lipopolysaccharide (LPS)-induced lymphoblasts. This immune response was more pronounced in x-irradiated mice and transferable via specific immune cells.
Area of Science:
- Immunology
- Cellular immunology
- Hypersensitivity reactions
Background:
- Delayed type hypersensitivity (DTH) is a critical immune response.
- Lymphoblasts are key cellular components in immune reactions.
- Understanding DTH mechanisms is vital for immune modulation.
Purpose of the Study:
- To investigate the induction of DTH using concanavalin A (Con A)-induced lymphoblasts.
- To assess the role of syngeneic, allogeneic, and xenogeneic lymphoblasts in DTH.
- To explore the influence of irradiation on DTH and identify mediating cells.
Main Methods:
- Induction of DTH in mice using syngeneic, allogeneic, and xenogeneic lymphoblasts.
- Measurement of DTH via footpad swelling and 125IudR accumulation.
- Cell transfer experiments using Thy-1+, nylon wool passed cells.
- Evaluation of lymphoblast-associated antigens versus contaminants.
Main Results:
- Syngeneic and allogeneic Con A-induced lymphoblasts elicited DTH responses upon challenge with LPS-induced lymphoblasts.
- Xenogeneic lymphoblasts induced a weaker DTH response.
- X-irradiation enhanced the DTH response, indicating control by irradiation-sensitive cells.
- DTH activity was transferable and linked to lymphoblast differentiation antigens.
Conclusions:
- Con A-induced lymphoblasts can induce DTH, with syngeneic and allogeneic cells being more effective than xenogeneic.
- Irradiation-sensitive cells play a regulatory role in DTH.
- The DTH response is mediated by specific cellular components of lymphoblasts, not by attached contaminants.