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Related Concept Videos

Phosphorylation01:02

Phosphorylation

55.7K
The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
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Microbial Biosensors01:17

Microbial Biosensors

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Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
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Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

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Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
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Photoluminescence: Applications01:14

Photoluminescence: Applications

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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Related Experiment Video

Updated: Apr 15, 2026

Assaying for Inorganic Polyphosphate in Bacteria
07:20

Assaying for Inorganic Polyphosphate in Bacteria

Published on: January 21, 2019

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Recent advances in phosphate biosensors.

Lata Sheo Bachan Upadhyay1, Nishant Verma

  • 1Department of Biotechnology, National Institute of Technology, Raipur, 492010, India, contactlataupadhyay@gmail.com.

Biotechnology Letters
|March 27, 2015
PubMed
Summary

Biosensors offer advanced phosphate analysis for environmental and biological monitoring. This review covers enzymatic and affinity-based biosensors, detailing techniques and sensitivity for clinical and environmental samples.

Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Environmental Science

Background:

  • Phosphate analysis is crucial due to its environmental and biological impacts.
  • Biosensors are key tools for detecting phosphate ions.
  • Enzymatic and affinity-based biosensors are prominent approaches.

Purpose of the Study:

  • To review recent advancements in biosensors for phosphate estimation.
  • To cover techniques and sensitivity in clinical and environmental samples.
  • To classify and compare biosensors based on enzyme usage.

Main Methods:

  • Review of enzymatic biosensors (single and multiple enzyme systems).
  • Analysis of non-enzymatic biosensors, including affinity-based types.
  • Classification and comparative analysis of biosensor systems.

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Main Results:

  • Biosensors provide sensitive detection of phosphate ions.
  • Enzymatic biosensors offer direct and indirect analysis.
  • Affinity-based biosensors present an alternative with high selectivity.

Conclusions:

  • Biosensor technology has significantly advanced phosphate estimation.
  • Diverse biosensor strategies are available for various applications.
  • Further development focuses on sensitivity and selectivity for phosphate detection.