Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

3.0K
The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
3.0K
Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

7.6K
When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
7.6K
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

11.3K
Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
11.3K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

7.7K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
7.7K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

2.2K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.2K
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

8.7K
Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for...
8.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rational Design of Crown Ether-Based Fluorescent Sensors for Group II Cations: Insights From Vibrational Spectroscopy and Computation.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same author

Unveiling Ciprofloxacin Protonation Isomers: an Integrated Approach with Mass Spectrometry, Ion Mobility Spectrometry and Infrared Ion Spectroscopy.

Journal of the American Society for Mass Spectrometry·2026
Same author

Oxidation Products of S-Adenosyl Methionine Probed by Infrared Multiple Photon Dissociation Spectroscopy.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same author

Mechanism of Aggregation of the NACore of α-Synuclein: Stable Oligomer Formation Competes with Fibril Formation with Implications for the Etiology of Parkinson's Disease.

Journal of the American Chemical Society·2026
Same author

Multi-modal structure characterization of synthetic batch impurities with liquid chromatography coupled to infrared ion spectroscopy.

The Analyst·2026
Same author

Gas-phase spectroscopy of H<sub>2</sub>O@C<sub>60</sub><sup>+</sup> and H<sub>2</sub>O@C<sub>60</sub>H<sup>+</sup> in the mid-infrared: the challenges of searching for endohedral fullerenes in space.

Physical chemistry chemical physics : PCCP·2026

Related Experiment Video

Updated: Apr 15, 2026

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
12:58

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

Published on: September 12, 2019

10.4K

IR Spectroscopic Techniques to Study Isolated Biomolecules.

Anouk M Rijs1, Jos Oomens

  • 1Institute for Molecules and Materials, FELIX Laboratory, Radboud University Nijmegen, Toernooiveld 7c, 6525 ED, Nijmegen, The Netherlands, a.rijs@science.ru.nl.

Topics in Current Chemistry
|March 28, 2015
PubMed
Summary
This summary is machine-generated.

Researchers combine mass spectrometry, infrared spectroscopy, and quantum calculations to study the structure of biological molecules. These methods offer detailed insights into molecular properties in a vacuum environment.

More Related Videos

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

10.0K
Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds
09:11

Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds

Published on: October 12, 2018

19.2K

Related Experiment Videos

Last Updated: Apr 15, 2026

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
12:58

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

Published on: September 12, 2019

10.4K
Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

10.0K
Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds
09:11

Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds

Published on: October 12, 2018

19.2K

Area of Science:

  • Physical Chemistry
  • Spectroscopy
  • Computational Chemistry

Background:

  • Studying biologically relevant molecules in a vacuum is crucial for understanding their intrinsic properties.
  • Traditional methods often lack the resolution to determine precise molecular structures.

Purpose of the Study:

  • To review experimental methods for investigating the structure of biologically relevant molecules in vacuo.
  • To highlight the synergy between mass spectrometry, infrared spectroscopy, and quantum-chemical calculations.

Main Methods:

  • Investigation of low-temperature neutral molecules in a molecular beam using double-resonance laser spectroscopy.
  • Spectroscopic analysis of ionized species via mass spectrometry and wavelength-dependent photo-dissociation.
  • Application of tunable infrared laser spectroscopy and quantum-chemical calculations.

Main Results:

  • Experimental infrared spectra provide detailed structural information.
  • Conformationally selective studies are possible for neutral molecules.
  • Ionized species can be effectively manipulated and analyzed.

Conclusions:

  • The combined approach of mass spectrometry, infrared action spectroscopy, and quantum-chemical calculations is powerful for elucidating molecular structures.
  • These techniques offer complementary insights into the properties of biological molecules in the gas phase.