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Author Spotlight: Investigating Viral Disruption of Intestinal Epithelial Signaling – Research Insights and Future Directions

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Recent progress in the development of genetically encoded Ca2+ indicators.

Kazuki Horikawa¹

¹Division for Bio-imaging, Support Center for Advanced Medical Sciences, Institute of Health Biosciences, the University of Tokushima Graduate School.

The Journal of Medical Investigation : JMI

|March 31, 2015

Summary

Genetically encoded calcium indicators (GECIs) offer advanced live imaging of calcium dynamics in cells. This review details GECI development, aiding researchers in selecting the best tool for their specific applications.

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Last Updated: Apr 15, 2026

Author Spotlight: Investigating Viral Disruption of Intestinal Epithelial Signaling – Research Insights and Future Directions

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Published on: January 19, 2024

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In vivo Neuronal Calcium Imaging in C. elegans

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Published on: April 10, 2013

Area of Science:

Biotechnology
Cell Biology
Neuroscience

Background:

Genetically encoded calcium indicators (GECIs) are crucial for observing intracellular calcium ion (Ca2+) fluctuations.
Advances in GFP technology and DNA engineering have led to the development of numerous GECI variants.

Purpose of the Study:

To review the historical development and recent advancements in GECIs.
To guide researchers in selecting optimal GECIs for diverse biological imaging applications.

Main Methods:

Literature review of GECI development and applications.
Analysis of GECI performance improvements over time.

Main Results:

A dozen sets of GECIs have been engineered, expanding their utility.
GECIs are now applicable at subcellular, single-cell, and population levels.
Significant improvements in GECI performance enhance live imaging capabilities.

Conclusions:

GECIs have evolved significantly, offering powerful tools for calcium imaging.
Understanding GECI progress facilitates informed selection for specific research needs.