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Updated: Apr 15, 2026
miRNA Expression Analyses in Prostate Cancer Clinical Tissues
Published on: September 8, 2015
[Expression of miR-223 in clear cell renal cell carcinoma and its significance]
1Department of Urology/State Key Laboratory of Kidney Diseases, General Hospital of PLA, Beijing 100853, China.E-mail: g4niuniu@163.com.
Objective:
To investigate the expression of miR-223 in clear cell renal cell carcinoma (ccRcc) and its clinical implications.
Methods:
Quantitative real-time PCR was employed to detect the levels of miR- 223 expression in ccRcc, pair-matched adjacent normal tissues and different renal cancer cell lines. Transwell migration essay and wound healing essay were used to evaluate the invasion and migration of renal cancer 786-O cells transfected with miR-223 mimics. MTT essay was used to measure the cell proliferation, and the cell cycle changes following the transfection were analyzed with flow cytometry.
Results:
Compared with the normal tissues, the cancer samples showed up-regulated miR-223 expression, which was associated with tumor size. In 786-O cell cultures, transfection with miR-223 mimics significantly enhanced cell migration (P<0.0001) and growth (P=0.006) and induced G1 cell cycle arrest.
Conclusion:
miR-223 promotes renal cancer cell migration and proliferation and may serve as a potential therapeutic target for ccRcc.
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