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Serial block face-scanning electron microscopy: a tool for studying embryonic development at the cell-matrix

Tobias Starborg1, Karl E Kadler

  • 1Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester, United Kingdom.

Birth Defects Research. Part C, Embryo Today : Reviews
|March 31, 2015
PubMed
Summary

Serial block face-scanning electron microscopy (SBF-SEM) enables detailed study of extracellular matrix (ECM) formation during later embryonic development. This advanced imaging technique overcomes limitations of traditional methods, revealing 3D tissue organization.

Keywords:
collagenelectron microscopyextracellular matrixfibrilstendon

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Microscopy

Background:

  • Early embryonic development is well-studied, but later stages involving extracellular matrix (ECM) formation remain poorly understood.
  • Conventional techniques struggle to analyze the complex 3D organization of ECM-rich tissues.
  • Significant knowledge gaps exist regarding the formation and structure of bulk tissues in adult vertebrates.

Purpose of the Study:

  • To review the emergence and application of Serial Block Face-Scanning Electron Microscopy (SBF-SEM) in developmental biology.
  • To highlight SBF-SEM's utility in studying the latter half of embryonic development.
  • To address the challenges in visualizing and understanding ECM formation and organization.

Main Methods:

  • Serial Block Face-Scanning Electron Microscopy (SBF-SEM) for high-resolution 3D imaging of biological tissues.
  • Application of SBF-SEM to study embryonic vertebrate development, focusing on ECM.
  • Comparison of SBF-SEM with conventional microscopy techniques.

Main Results:

  • SBF-SEM provides sufficient resolution to study the organization of the ECM within large tissue volumes containing numerous cells.
  • The technique is becoming routine for high-end in vivo studies of embryonic structures.
  • SBF-SEM overcomes the limitations of traditional methods for studying ECM.

Conclusions:

  • SBF-SEM is a powerful and increasingly accessible tool for investigating embryonic vertebrate development.
  • It offers unprecedented insights into the 3D organization and formation of ECM during later embryonic stages.
  • This technology is crucial for filling knowledge gaps in understanding tissue development.