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Updated: Apr 15, 2026

09:17
Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay
Published on: January 11, 2017
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4-Phosphopyrazol-2-yl alanine: a non-hydrolysable analogue of phosphohistidine
Matthew Lilley1, Bezaleel Mambwe, Mark J Thompson
1Department of Chemistry, The University of Sheffield, Dainton Building, Sheffield, S3 7HF, UK. r.f.w.jackson@shef.ac.uk.
Abstract:
We report the synthesis of a stable analogue of τ-phosphohistidine: 4-phosphopyrazol-2-yl alanine (pPza). Polyclonal antibodies generated against the mimic show high reactivity and selectivity for τ-phosphohistidine, with minor or no cross-reactivity towards non-phosphorylated histidine or O-phosphoamino acids, including phosphotyrosine.

