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Updated: Apr 15, 2026

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

Principles of protein labeling techniques.

Christian Obermaier¹, Anja Griebel, Reiner Westermeier

¹SERVA Electrophoresis GmbH, Carl-Benz-Straße 7, Heidelberg, 69115, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|March 31, 2015

Summary

This summary is machine-generated.

Protein labeling techniques are essential for proteomic profiling, utilizing stable isotopes, mass tags, or fluorophores for enhanced detection and quantification. These methods simplify workflows and improve sensitivity in analyzing biological samples.

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Last Updated: Apr 15, 2026

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Area of Science:

Proteomics
Biochemistry
Analytical Chemistry

Background:

Protein labeling is crucial for advanced proteomic profiling.
Various tagging strategies exist, including stable isotopes, mass tags, and fluorophores.
These tags enable diverse analytical approaches like mass spectrometry and fluorescence imaging.

Purpose of the Study:

To review and describe protein labeling methods for proteomic analysis.
To discuss the principles, modifications, benefits, and drawbacks of different labeling approaches.
To highlight the utility of protein labeling in monitoring biological processes and quantifying compounds.

Main Methods:

Stable isotope labeling (SIL) during cell growth.
Mass tagging for mass spectrometry detection.
Fluorophore labeling for fluorescence imaging.
Chemical modification of lysine, N-terminus, or cysteine residues.

Main Results:

Stable isotope and mass tag-labeled proteins are analyzed via mass spectrometry.
Fluorescently labeled proteins are detected using fluorescence imagers.
Labeling enables multiplexed sample analysis, improved detection sensitivity, and simplified workflows.
Methods allow for monitoring biological processes, quantifying compounds, and detecting protein modifications.

Conclusions:

Protein labeling is indispensable for comprehensive proteomic profiling.
The choice of labeling method depends on the specific analytical goals and sample type.
Understanding the benefits and shortcomings of each method optimizes experimental design.