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Affinity Chromatography

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...

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Updated: Apr 15, 2026

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

Amyloid-binding proteins: affinity-based separation, proteomic identification, and optical biosensor validation.

Alexei Medvedev¹, Olga Buneeva, Arthur Kopylov

¹Department of Proteomic Research and Mass Spectrometry, Institute of Biomedical Chemistry, Moscow, Russia, professor57@yandex.ru.

Methods in Molecular Biology (Clifton, N.J.)

|March 31, 2015

Summary

This summary is machine-generated.

Researchers developed a new method to identify proteins that bind to amyloid-beta within the brain. This study focuses on understanding intracellular amyloid-beta interactions relevant to Alzheimer's disease (AD) progression.

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Last Updated: Apr 15, 2026

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Published on: April 1, 2017

Peptide-based Identification of Functional Motifs and their Binding Partners

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

Area of Science:

Neuroscience
Proteomics
Biochemistry

Background:

Amyloid-beta peptide is implicated in Alzheimer's disease (AD) pathogenesis.
Intracellular accumulation of amyloid-beta is observed in AD.
Proteins binding to intracellular amyloid-beta are not well understood.

Purpose of the Study:

To develop a protocol for profiling amyloid-beta-binding proteins in the rat brain.
To identify and validate these intracellular binding proteins.
To enhance understanding of molecular mechanisms in AD.

Main Methods:

Affinity-based profiling using a beta-amyloid sorbent.
Proteomic identification of bound proteins.
Validation using surface plasmon resonance (SPR) analysis.
Optimization of beta-amyloid immobilization conditions for SPR.

Main Results:

A robust protocol for selecting amyloid-beta immobilization conditions and resin was established.
The method enabled the identification of specific amyloid-beta-binding proteins.
Surface plasmon resonance confirmed the binding interactions.

Conclusions:

The developed protocol is effective for identifying intracellular amyloid-beta-binding proteins.
This work provides a foundation for further investigation into the role of these proteins in AD.
Characterizing these interactions is crucial for understanding AD pathology.