Related Experiment Video
Updated: Apr 15, 2026
Induction of Adhesion-dependent Signals Using Low-intensity Ultrasound
Published on: May 8, 2012
Low-intensity pulsed ultrasound enhances bone formation around miniscrew implants
Khaliunaa Ganzorig1, Shingo Kuroda2, Yuichi Maeda1
1Department of Orthodontics and Dentofacial Orthopedics, Tokushima University Graduate School of Oral Sciences, Tokushima, Japan.
Unlabelled:
Miniscrew implants (MSIs) are currently used to provide absolute anchorage in orthodontics; however, their initial stability is an issue of concern. Application of low-intensity pulsed ultrasound (LIPUS) can promote bone healing. Therefore, LIPUS application may stimulate bone formation around MSIs and enhance their initial stability.
Aim:
To investigate the effect of LIPUS exposure on bone formation after implantation of titanium (Ti) and stainless steel (SS) MSIs.
Methods:
MSIs made of Ti-6Al-4V and 316L SS were placed on rat tibiae and treated with LIPUS. The bone morphology around MSIs was evaluated by scanning electron microscopy and three-dimensional micro-computed tomography. MC3T3-E1 cells cultured on Ti and SS discs were treated with LIPUS, and the temporary expression of alkaline phosphatase (ALP) was examined.
Results:
Bone-implant contact increased gradually from day 3 to day 14 after MSI insertion. LIPUS application increased the cortical bone density, cortical bone thickness, and cortical bone rate after implantation of Ti and SS MSIs (P<0.05). LIPUS exposure induced ALP upregulation in MC3T3-E1 cells at day 3 (P<0.05).
Conclusion:
LIPUS enhanced bone formation around Ti and SS MSIs, enhancing the initial stability of MSIs.
