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Updated: Apr 15, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
Published on: April 4, 2025
Development of fluorescent probes specific for parallel-stranded G-quadruplexes by a library approach
Liyun Zhang1, Jun Cheng Er, Xin Li
1Institute of Technical Biology and Agriculture Engineering, Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, P. R. China. zly0605@ustc.edu.cn.
Abstract:
A 241-membered cyanine-based library was constructed by the combinatorial chemistry strategy. Combined with high-throughput screening, we successfully discovered a novel fluorescent probe (CyC-M716) capable of identifying a subset of parallel G-quadruplexes with propeller loops stretching across three tetrad layers with high sensitivity and selectivity.
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