Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Finding the hidden genes in Leishmania guyanensis genome: Application of proteogenomics approach to improve the genome annotation in the kinetoplastid parasite.

Acta tropica·2025

Same author

Identification of new protein-coding potential in Leishmania braziliensis using a proteogenomics approach.

Biochimica et biophysica acta. Proteins and proteomics·2025

Same author

Response of human iPSC-cardiomyocytes to adrenergic drugs assessed by high-throughput pericellular oxygen measurements.

bioRxiv : the preprint server for biology·2025

Same author

Pericellular oxygen dynamics in human cardiac fibroblasts and iPSC-cardiomyocytes in high-throughput plates: insights from experiments and modeling.

bioRxiv : the preprint server for biology·2025

Same author

Regulatory Considerations for Development of Wound Dressing Devices Used for Chemical and Radiation Injuries.

Disaster medicine and public health preparedness·2024

Same author

A microfluidic cover converts a standard 96-well plate into a mass-transport-controlled immunoassay system.

Biomicrofluidics·2024

Same journal

Parallelized contactless microfluidic dispenser with superhydrophobic nozzles for scalable combinatorial screening.

Biomicrofluidics·2026

Same journal

Time resolved luminescence of millisecond lifetime dyes in droplet microfluidic systems.

Biomicrofluidics·2026

Same journal

Emerging trends in functional molecularly imprinted polymers for electrochemical detection of biomarkers.

Biomicrofluidics·2025

Same journal

Deep learning assisted mechanotyping of individual cells through repeated deformations and relaxations in undulating channels.

Biomicrofluidics·2025

Same journal

<i>Giardia</i> purification from fecal samples using rigid spiral inertial microfluidics.

Biomicrofluidics·2025

Same journal

Point of care sepsis diagnosis: Exploring microfluidic techniques for sample preparation, biomarker isolation, and detection.

Biomicrofluidics·2025

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 15, 2026

A Microfluidic-based Hydrodynamic Trap for Single Particles

A Microfluidic-based Hydrodynamic Trap for Single Particles

Published on: January 21, 2011

Streamline based design guideline for deterministic microfluidic hydrodynamic single cell traps.

Allan Guan¹, Aditi Shenoy¹, Richard Smith¹

¹Department of Biomedical Engineering, The George Washington University , Washington, District of Columbia 20052, USA.

Biomicrofluidics

|April 1, 2015

Summary

This summary is machine-generated.

This study introduces a new analytical guideline for hydrodynamic trapping in microfluidic devices, optimizing cell capture efficiency by considering cell size for deterministic single cell isolation. Experiments confirm 100% capture rates for specific particle sizes.

More Related Videos

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

Published on: September 27, 2019

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

Related Experiment Videos

Last Updated: Apr 15, 2026

A Microfluidic-based Hydrodynamic Trap for Single Particles

A Microfluidic-based Hydrodynamic Trap for Single Particles

Published on: January 21, 2011

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

Published on: September 27, 2019

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

Area of Science:

Biotechnology
Microfluidics
Cell Biology

Background:

Single cell studies require efficient cell capture and isolation.
Microfluidic hydrodynamic trapping is simple and scalable but lacks quantitative design criteria.
Current methods often result in probabilistic, non-optimal cell trapping.

Purpose of the Study:

To develop an analytical design guideline for deterministic single cell hydrodynamic trapping.
To optimize streamline distributions considering cell size as a key parameter.
To achieve high capture efficiency in microfluidic devices.

Main Methods:

Developed an analytical guideline for hydrodynamic trapping based on first principles.
Utilized finite element modeling to determine optimal design parameters.
Validated the design with on-chip microbead and white blood cell trapping experiments.

Main Results:

Demonstrated a design guideline for deterministic single cell hydrodynamic trapping.
Achieved 100% capture efficiency for a specific particle size.
Experimental validation confirmed simulation predictions.

Conclusions:

The developed analytical guideline provides quantitative criteria for optimal microfluidic cell trap design.
This approach enables deterministic and highly efficient single cell isolation.
The method is applicable to various cell types and microfluidic applications.