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Updated: Apr 15, 2026
Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules
Published on: December 16, 2021
An ultrasensitive nano UHPLC-ESI-MS/MS method for the quantification of three neuromedin-like peptides in
Katrien Maes1, Guillaume Béchade, Jolien Van Schoors
1Department of Pharmaceutical Chemistry & Drug Analysis, Center for Neurosciences (C4N), Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium.
Aim:
An ultrasensitive nano UHPLC-ESI-MS/MS method is developed to simultaneously monitor three low-concentration neuromedin-like peptides in microdialysates.
Results:
Peptide preconcentration and sample desalting is performed online on a trap column. A shallow gradient slope at 300 nl/min on the analytical column maintained at 35°C, followed by two saw-tooth column wash cycles, results in the highest sensitivity and the lowest carryover. The validated method allows the accurate and precise quantification of 0.5 pM neurotensin and neuromedin N (2.5 amol on column), and of 3.0 pM neuromedin B (15.0 amol on column) in in vivo microdialysates without the use of internal standards.
Conclusion:
The assay is an important tool for elucidating the role of these neuromedin-like peptides in the pathophysiology of neurological disorders.
