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Intranasal Delivery of Therapeutic Stem Cells to Glioblastoma in a Mouse Model
Published on: June 4, 2017
Neural stem cell-mediated delivery of oncolytic adenovirus
Julius W Kim1, J Robert Kane1, Jacob S Young1
1The Brain Tumor Center, University of Chicago, Chicago, Illinois.
Abstract:
The use of stem cells (SCs) as carriers for therapeutic agents has now progressed to early clinical trials. These clinical trials exploring SC-mediated delivery of oncolytic adenoviruses will commence in the near future, hopefully yielding meritorious results that can provoke further scientific inquiry. Preclinical animal studies have demonstrated that SCs can be successfully loaded with conditionally-replicative adenoviruses and delivered to the tumor, whereupon they may evoke pronounced therapeutic efficacy. In this protocol, we describe the maintenance of SCs, provide an analysis of optimal adenoviral titers for SC loading, and evaluate the optimized viral loading on SCs.
Insights
Stem cells (SCs) are being explored as carriers for oncolytic adenoviruses in early clinical trials. Preclinical studies show SCs can effectively deliver these viruses to tumors, demonstrating promising therapeutic potential for cancer treatment.
Area of Science:
- * Oncolytic virotherapy
- * Stem cell biology
- * Gene therapy
Background:
- * Stem cells (SCs) show potential as delivery vehicles for therapeutic agents.
- * SC-mediated delivery of oncolytic adenoviruses is advancing towards clinical applications.
- * Preclinical research indicates successful SC loading and tumor delivery of these viruses.
Purpose of the Study:
- * To detail a protocol for stem cell (SC) maintenance.
- * To analyze optimal adenoviral titers for SC loading.
- * To evaluate the efficiency of viral loading onto SCs for therapeutic delivery.
Main Methods:
- * Stem cell culture and maintenance protocols.
- * Titration of adenoviral vectors.
- * Quantification of viral load on stem cells.
Main Results:
- * Established protocols for SC maintenance.
- * Determined optimal adenoviral titers for effective SC loading.
- * Demonstrated successful and efficient loading of adenoviruses onto SCs.
Conclusions:
- * The described protocol facilitates SC loading with oncolytic adenoviruses.
- * Optimized viral loading on SCs is crucial for potential therapeutic efficacy.
- * This work supports the progression of SC-mediated oncolytic adenovirus therapy into clinical trials.
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