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Related Concept Videos

Atomic Force Microscopy01:08

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
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Related Experiment Video

Updated: Apr 15, 2026

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging
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Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

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Chromatin imaging with time-lapse atomic force microscopy.

Yuri L Lyubchenko1, Luda S Shlyakhtenko

  • 1Department of Pharmaceutical Sciences, University of Nebraska Medical Center, 986025, Nebraska Medical Center, Omaha, NE, 68198-6025, USA, ylyubchenko@unmc.edu.

Methods in Molecular Biology (Clifton, N.J.)
|April 2, 2015
PubMed
Summary
This summary is machine-generated.

Time-lapse atomic force microscopy (AFM) visualizes nanoscale biological dynamics. This chapter details protocols for studying nucleosome structure and dynamics using high-speed AFM, including sample preparation.

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Area of Science:

  • Biophysics
  • Molecular Biology
  • Nanotechnology

Background:

  • Time-lapse atomic force microscopy (AFM) enables nanoscale visualization of biological systems.
  • High-speed AFM allows imaging of protein and protein-DNA complex dynamics in milliseconds.

Purpose of the Study:

  • To describe protocols for studying nucleosome structure and dynamics using time-lapse AFM.
  • To provide specifics for chromatin sample preparation for AFM imaging.

Main Methods:

  • Utilizing time-lapse atomic force microscopy (AFM).
  • Employing high-speed AFM instrumentation.
  • Detailed protocols for surface and chromatin sample preparation.

Main Results:

  • Established protocols for AFM-based nucleosome studies.
  • Demonstrated feasibility of high-speed AFM for millisecond-range dynamics.
  • Provided comprehensive sample preparation guidelines.

Conclusions:

  • Time-lapse AFM, particularly high-speed AFM, is effective for investigating nucleosome dynamics.
  • Standardized protocols enhance the study of chromatin structure and function.
  • Optimized sample preparation is crucial for successful AFM imaging.