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Related Concept Videos

Histone Modification02:32

Histone Modification

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The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
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Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
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Assays for Validating Histone Acetyltransferase Inhibitors
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Histone deacetylase activity assay.

Lirong Peng1, Zhigang Yuan, Edward Seto

  • 1Molecular Oncology Department, H. Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL, 33612, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 2, 2015
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Summary
This summary is machine-generated.

Histone deacetylases (HDACs) are enzymes crucial for cellular processes. This chapter details reliable assays for measuring HDAC enzymatic activity, vital for understanding disease and biological functions.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • Histone deacetylases (HDACs) remove acetyl groups from histones and nonhistone proteins.
  • HDACs regulate critical cellular functions like gene transcription, differentiation, DNA repair, and apoptosis.
  • Dysregulation of HDACs is linked to various human diseases.

Purpose of the Study:

  • To provide updated, user-friendly methods for assaying HDAC enzymatic activities.
  • To establish consistent and reliable techniques for HDAC analysis.

Main Methods:

  • The chapter focuses on presenting established and novel approaches for HDAC activity assays.
  • Detailed protocols and techniques are described for accurate measurement.

Main Results:

  • The presented assays enable precise quantification of HDAC enzymatic function.
  • These methods facilitate the study of HDACs in both normal and pathological conditions.

Conclusions:

  • Accurate HDAC assays are essential for research into HDAC-related diseases.
  • The chapter offers valuable tools for scientists studying HDACs and their biological roles.