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Updated: Feb 28, 2026

Estimation of Telomeric Repeat-containing RNA from DNA/RNA Hybrid Complexes

Estimation of Telomeric Repeat-containing RNA from DNA/RNA Hybrid Complexes

Published on: December 5, 2025

A hybrid chimeric system for versatile and ultra-sensitive RNase detection.

Stefano Persano^1,2, Giuseppe Vecchio¹, Pier Paolo Pompa¹

¹Istituto Italiano di Tecnologia, Center for Bio-Molecular Nanotechnologies@UniLe, Via Barsanti, 73010 Arnesano (Lecce), Italy.

Scientific Reports

|April 2, 2015

Summary

We developed a sensitive new assay for detecting RNases using DNAzyme-activated fluorescent probes. This method enhances detection sensitivity for various RNase classes in biological samples.

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Published on: October 6, 2022

Area of Science:

Biochemistry
Molecular Biology
Analytical Chemistry

Background:

Ribonucleases (RNases) are crucial enzymes involved in RNA metabolism and degradation.
Accurate detection of RNase activity is vital for understanding biological processes and disease states.
Existing RNase detection assays often lack sensitivity or versatility.

Purpose of the Study:

To develop a highly sensitive and versatile strategy for detecting multiple classes of RNases.
To establish a platform for screening RNase inhibitors and analyzing RNase activity in biological samples.

Main Methods:

A two-step approach utilizing DNA-RNA-DNA chimeric Hairpin Probes (cHP) conjugated to magnetic microparticles.
Incorporation of a DNAzyme sequence within the cHP for catalytic signal amplification.
Use of molecular beacons for fluorescence-based signal generation upon DNAzyme activity.

Main Results:

The assay demonstrated significantly higher sensitivity for detecting RNase A, E. coli RNase I, and RNase H compared to existing methods.
Successfully analyzed the inhibitory effects of penicillin and streptomycin on RNase H activity.
Enabled direct detection of RNase activity in crude biological samples like blood and saliva, and in cell culture medium.

Conclusions:

The developed strategy offers a sensitive, versatile, and cost-effective tool for RNase detection.
This method is applicable for inhibitor screening and monitoring RNase levels in various biological contexts.
The assay's high sensitivity and broad applicability make it valuable for research and diagnostics.