Maximizing the diagnostic yield from bone marrow aspirate material using the cell block technique on clot sections
Menchu G Ong1, Mary Lowery-Nordberg2, Sudha Pillarisetti3
1Department of Pathology, Louisiana State University Health Sciences Center, Shreveport, LA.
Laboratory Medicine
|April 2, 2015
Summary
The novel cell block technique maximizes bone marrow aspirate yield, ensuring adequate diagnostic tissue. This method is crucial for accurate morphological interpretation and molecular studies when core biopsies are insufficient.
Area of Science:
- Hematopathology
- Oncology
- Diagnostic Pathology
Background:
- Bone marrow aspirate clot sections are vital for diagnosis.
- Improper acquisition leads to insufficient tissue and wasted resources.
Purpose of the Study:
- To present a novel cell block method for bone marrow aspirate.
- To ensure maximum tissue yield for diagnostic accuracy.
Main Methods:
- Evaluation of paraffin-embedded hematopoietic tissue from bone marrow aspirate using the cell block method.
Main Results:
- Adequate clot sections allow diagnoses comparable to core biopsies.
- Includes diagnoses via H&E, IHC, FISH, and PCR.
Conclusions:
- Bone marrow clot sections lacking adequate tissue are diagnostically insufficient.
- The cell block technique is recommended for optimal material capture and diagnosis.
