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INCA: software for consort 30 analysis of flow cytometric calcium determinations
J F Keij1, A W Griffioen, T H The
1Department of Clinical Immunology, University of Groningen, The Netherlands.
Cytometry
|November 1, 1989
Abstract:
The INCA program converts Consort 30-generated fluorescence list mode data collected from Indo-1-stained cells to absolute intracellular calcium concentrations (nM Ca2+i). The calcium data are plotted vs. time, allowing the user to analyze the fractions of cells responding to a given stimulus. Converted files can be restored to disk after replacing FL1 and FL2 with time and calcium, respectively, for future analysis.