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Updated: Apr 15, 2026
A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering
Published on: December 7, 2016
Comparative analysis of cellulose acetate hemoglobin electrophoresis and high performance liquid chromatography for
Shafi Mohammad Khosa1, Muhammad Usman1, Moinuddin Moinuddin2
1Baqai Institute of Hematology, Baqai Medical University, Karachi, Pakistan. ; Baqai Institute of Hematology, Baqai Medical University, Karachi, Pakistan.
Background:
The present study is designed to evaluate the reliability and cost effectiveness of cellulose acetate Hb electrophoresis and high performance liquid chromatography (HPLC) in the determination of HbA2 levels.
Methods:
The test population comprised 160 individuals divided into four groups: normal individuals, β-thalassemia trait (BTT) patients, iron deficiency anemia (IDA) patients, and co-morbid patients (BTT with IDA). HbA2 levels determined using cellulose acetate Hb electrophoresis and HPLC were compared.
Results:
HbA2 levels were found to be diagnostic for classical BTT using either method. In co-morbid cases, both techniques failed to diagnose all cases of BTT. The sensitivity, specificity, and Youden's index for detection of the co-morbid condition was 69% and 66% for HPLC and cellulose acetate Hb electrophoresis, respectively.
Conclusion:
This study revealed that semi-automated cellulose acetate Hb electrophoresis is more suitable for use in β-thalassemia prevention programs in low-income countries like Pakistan. This technique is easily available, simple and cost effective.
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