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Mulberry leaf polysaccharides modulate murine bone-marrow-derived dendritic cell maturation.

Ming Xue1, Haiyan Sun, Yan Cao

  • 1a Department of Endodontics ; School of Stomatology; China Medical University ; Shenyang , China.

Human Vaccines & Immunotherapeutics
|April 2, 2015
PubMed
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Mulberry leaf polysaccharides (MLPs) were found to positively modulate murine bone-marrow-derived dendritic cells (BMDCs). This study shows MLPs enhance dendritic cell maturation, impacting immune responses.

Area of Science:

  • Immunology
  • Natural Products Chemistry

Background:

  • Mulberry leaf components, including iminosugars, flavonoids, and polysaccharides, exhibit anti-diabetic properties.
  • Dendritic cells (DCs) are crucial immune cells that bridge innate and adaptive immunity.
  • Modulating DC function is a key strategy for immune system regulation.

Purpose of the Study:

  • To investigate the immunomodulatory effects of mulberry leaf polysaccharides (MLPs) on murine bone-marrow-derived dendritic cells (BMDCs).
  • To assess how MLPs influence the maturation and function of BMDCs.

Main Methods:

  • Transmission electron microscopy (TEM) for ultrastructural analysis.
  • Flow cytometry (FCM) for phenotype analysis of maturation markers (CD80, CD86, CD83, CD40, MHC II).
  • Enzyme-linked immunosorbent assay (ELISA) to measure phagocytosis, acid phosphatase (ACP) activity, and cytokine production (IL-12, TNF-α).
Keywords:
ACP, acidic phosphataseBMDCs, bone-marrow-derived dendritic cellsDAB, 3, 3′-diaminobenzidineFCM, flow cytometryLPS, lipopolysaccharideMACS, magnetic activated cell sortingMLP, mulberry leaf polysaccharidesMTS, methyl tolyl sulfideTEM, transmission electron microscopybone-marrow-derived dendritic cellsimmunoregulationmaturationmulberry leaf polysaccharides

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Main Results:

  • MLPs significantly induced BMDC maturation.
  • Upregulation of key surface markers (CD80, CD86, CD83, CD40, MHC II) was observed.
  • Phagocytosis and ACP activity were downregulated, while IL-12 and TNF-α production were enhanced.

Conclusions:

  • Mulberry leaf polysaccharides (MLPs) demonstrate a positive modulatory effect on dendritic cell maturation and function.
  • MLPs can enhance the immune-activating capacity of dendritic cells.
  • These findings suggest potential therapeutic applications of MLPs in immune modulation.