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Related Experiment Video

Updated: Jul 6, 2026

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

Published on: September 6, 2014

Early reprogramming regulators identified by prospective isolation and mass cytometry.

Ernesto Lujan¹, Eli R Zunder², Yi Han Ng³

¹1] Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, California 94305, USA [2] Department of Genetics, Stanford University, Stanford, California 94305, USA [3] Department of Pathology, Stanford University, Stanford, California 94305, USA.

|April 2, 2015

Summary

Researchers identified novel surface markers (CD73, CD49d, CD200) on early reprogramming-prone cells. These transient intermediates are crucial for induced pluripotent stem (iPS) cell generation and understanding pluripotency acquisition.

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Area of Science:

Stem cell biology
Cellular reprogramming
Epigenetics

Background:

Induced pluripotent stem (iPS) cell reprogramming involves heterogeneous intermediates.
Identifying these intermediates is key to understanding reprogramming mechanisms.
Previous marker assumptions for intermediates were inaccurate.

Purpose of the Study:

To identify novel surface markers for early reprogramming intermediates.
To decipher the initial steps and regulatory mechanisms of iPS cell induction.
To characterize transient cell states during reprogramming.

Main Methods:

Systematic functional surface marker screening.
Single-cell mass cytometry and prospective cell isolation.
Gene expression profiling to identify key regulators.

Main Results:

Early reprogramming-prone cells express unique markers (CD73, CD49d, CD200), distinct from fibroblasts and iPS cells.
These markers identify transient intermediates bridging donor cell silencing and pluripotency.
Early upregulation of Nr0b1 and Etv5 precedes pluripotency gene activation and is essential for reprogramming.

Conclusions:

CD73, CD49d, and CD200 identify early reprogramming intermediates.
Nr0b1 and Etv5 are critical early regulators required for iPS cell induction.
This study elucidates the initial hierarchical events in pluripotency acquisition.